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Protein Extraction
Catalog Number:
(ENZOENZ450150100)
Supplier:
ENZO LIFE SCIENCES
Description:
Rapid, reliable method to isolate nucleic acid-free nuclear proteins in mammalian cells and tissues.
UOM:
1 * 1 KIT
New Product
Catalog Number:
(ENZOENZ450130002)
Supplier:
ENZO LIFE SCIENCES
Description:
Rapid, reliable, high throughput method to isolate histone proteins in mammalian cells for histone modification applications.
UOM:
1 * 1 KIT
New Product
Catalog Number:
(786-260)
Supplier:
G-Biosciences
Description:
FOCUS™ SubCell for the fractionation of mitochondrial, nuclear, and cytosolic soluble and membrane proteins.
UOM:
1 * 1 KIT
Catalog Number:
(786-241)
Supplier:
G-Biosciences
Description:
InGel™ provides a reliable method for the proteolytic digestion and subsequent extration of silver stained proteins in gel for subsequent analysis by mass spectrometry.
UOM:
1 * 1 KIT
Catalog Number:
(786-250)
Supplier:
G-Biosciences
Description:
Lipid rafts are membrane microdomains that are enriched in caveolin, cholesterol, glycolipids, sphingolipids and glycosyl-phosphatidylinositol. Lipid rafts are also known as detergent-insoluble glycolipid-enriched complexes (GEMs) or DIGs. Many signalling proteins, including glycosylphosphatidylinositol (GPI)-anchored proteins, doubly-acylated tyrosine kinases of the Src family, and transmembrane proteins, are located in lipid rafts. Lipid raft localised proteins have been shown to be involved in intracellular membrane trafficking and signalling.
UOM:
1 * 1 KIT
Supplier:
G-Biosciences
Description:
Protein-S-S-Reductant™ uses TCEP (Tris [2-carboxyethyl] phosphine), a popular alternative to β-mercaptoethanol and DTT (dithiothreitol). TCEP improves stability, increases effectiveness, and reduces proteins over a wider range of pH, including lower acidic pHs. Protein-S-S-Reductant™ completely reduces stable disulfide bonds in less than 5 minutes at room temperature and is compatible with the protein alkylation reactions. This ready to use solution is at a neutral pH and stabilised for long-term storage. Simply supplement Protein-S-S-Reductant™ in place of DTT or β-mercaptoethanol and boil the sample.
Supplier:
Biotium
Description:
AccuOrange™ Protein Quantitation Kit is a highly sensitive fluorescence-based assay for quantitating purified protein samples in 96-well format (excitation/emission = 480/598 nm).
Supplier:
Thermo Fisher Scientific
Description:
Pierce™ TMT™ Isobaric Mass Tagging kits and reagents enable multiplex quantitation of proteins extracted from cells and tissues using tandem mass spectrometry. The Tandem Mass Tag (TMT) reagent family consists of TMTduplex and TMTsixplex sets which are specially designed to enable a rapid and cost-effective transition from method development to high-throughput protein quantitation.
Catalog Number:
(786-259)
Supplier:
G-Biosciences
Description:
Specifically designed for plant research and supplied with plant specific reagents, including reagents for removal of plant pigments and other natural products that may interfere with protein analysis. Extracts and solubilises nearly all of the proteins from plants, including membrane as well as soluble proteins. Supplied with a strong proprietary chaotropic extraction buffer to solubilise even the most difficult proteins.
UOM:
1 * 1 KIT
Catalog Number:
(786-360)
Supplier:
G-Biosciences
Description:
Extracts and solubilises nearly all of the proteins from insect cell cultures (i.e. Sf9 and Sf21), including membrane as well as soluble proteins, using a strong chaotropic extraction buffer to solubilise even the most difficult proteins.
UOM:
1 * 1 KIT
Catalog Number:
(ENZOBMLKI1761250)
Supplier:
ENZO LIFE SCIENCES
Description:
FLUOR DE LYS® Developer II is an essential reagent for signal generation in assays done with the FLUOR DE LYS®-HDAC8, FLUOR DE LYS®-SIRT1 and FLUOR DE LYS®-SIRT2 substrates. Deacetylation of the FLUOR DE LYS® substrate sensitises it so that, in a second step, treatment with the FLUOR DE LYS® Developer II produces a fluorophore. Typically, an ‘inhibitor stop’ will be included in the 1X Developer dilution (e.g. nicotinamide for sirtuins).
UOM:
1 * 1,25 mL
New Product
Supplier:
Thermo Fisher Scientific
Description:
The Pierce™ Universal Nuclease for Cell Lysis is ideal for a wide variety of applications where complete digestion of nucleic acids is needed when preparing cell lysates.
Catalog Number:
(786-249)
Supplier:
G-Biosciences
Description:
FOCUS™ Membrane Proteins for the fractionation of highly enriched membrane proteins.
UOM:
1 * 1 KIT
Catalog Number:
(786-257)
Supplier:
G-Biosciences
Description:
Specifically designed for yeast research and supplied with yeast specific reagents. Extracts and solubilises nearly all of the proteins from yeast, including membrane and soluble proteins. Extraction is based on gentle lysis of yeast cells with a yeast lytic enzyme preparation, LongLife™ Zymolyase®, which has improved stability and shelf life. Enzymatic action is followed by extraction of total proteome with the supplied strong chaotropic extraction buffer that solubilises even the most difficult proteins.
UOM:
1 * 1 KIT
Catalog Number:
(786-244)
Supplier:
G-Biosciences
Description:
SilverOUT removes interfering silver ions from gel pieces.
UOM:
1 * 100 Tests
Supplier:
G-Biosciences
Description:
Antibody Fab and F(ab)₂ Fragmentation of Mouse IgG<sub>1</sub> Kits are designed for the generation and isolation of Fab and F(ab)₂ fragments from mouse IgG₁ molecules. The kit utilises Immobilised Ficin resin. Ficin (or Ficain) (~25000 Da) is a cysteine protease enzyme (EC 3.4.22.3) isolated from fig latex that has the endopeptidase activity to cleave immunoglobulin G molecules in the hinge region. Ficin has an effective range of pH 4 to 9,5 with an optimal pH of 6,5 and cleaves bonds that involve uncharged or aromatic amino acids. Ficin is typically used to cleave mouse IgG₁ as this is difficult to cleave with papain and pepsin. In the presence of 1 to 4 mM or 10 to 20 mM cysteine, ficin generates F(ab)₂ and Fab fragments respectively.
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 is still displayed and you need assistance, please call us on 0800 22 33 44.
Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the
is still displayed and you need assistance, please call us on 0800 22 33 44
Additional Documentation may be needed to purchase this item. A VWR representative will contact you if needed.
Additional Documentation may be needed to purchase this item. A VWR representative will contact you if needed.
This product has been blocked by your organisation. Please contact your purchasing department for more information.
The original product is no longer available. The replacement shown is available.
Product(s) marked with this symbol are discontinued - sold till end of stock. Alternatives may be available by searching with the VWR Catalogue Number listed above. If you need further assistance, please call VWR Customer Service on 0800 22 33 44.
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